Rat Cytokines Search Results


rat cytokine inflammation 9 plex elisa kit  (Quansys Biosciences)
92
Quansys Biosciences rat cytokine inflammation 9 plex elisa kit
Rat Cytokine Inflammation 9 Plex Elisa Kit, supplied by Quansys Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pm34224822-79-6-12?v=Quansys+Biosciences
Average 92 stars, based on 1 article reviews
rat cytokine inflammation 9 plex elisa kit - by Bioz Stars, 2026-08
92/100 stars
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rat proteome profiler cytokine array kit  (R&D Systems)
95
R&D Systems rat proteome profiler cytokine array kit
Rat Proteome Profiler Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pm39001671-185-18-27?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
rat proteome profiler cytokine array kit - by Bioz Stars, 2026-08
95/100 stars
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rat specific proteome profiler array  (R&D Systems)
95
R&D Systems rat specific proteome profiler array
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Rat Specific Proteome Profiler Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/10__1074_slash_jbc__m110__150847-85-6-14?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
rat specific proteome profiler array - by Bioz Stars, 2026-08
95/100 stars
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protein chip  (R&D Systems)
95
R&D Systems protein chip
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Protein Chip, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pmc10272649-104-7-16?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
protein chip - by Bioz Stars, 2026-08
95/100 stars
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proteome profilertm rat xl cytokine array kit  (R&D Systems)
94
R&D Systems proteome profilertm rat xl cytokine array kit
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Proteome Profilertm Rat Xl Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pm32042319-79-6-16?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
proteome profilertm rat xl cytokine array kit - by Bioz Stars, 2026-08
94/100 stars
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il 10  (Boster Bio)
93
Boster Bio il 10
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Il 10, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/10__2147_slash_jir__s592917-118-16-18?v=Boster+Bio
Average 93 stars, based on 1 article reviews
il 10 - by Bioz Stars, 2026-08
93/100 stars
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elisa kits  (Boster Bio)
90
Boster Bio elisa kits
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pmc13068612-61-0-4?v=Boster+Bio
Average 90 stars, based on 1 article reviews
elisa kits - by Bioz Stars, 2026-08
90/100 stars
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il 10 concentrations  (Boster Bio)
94
Boster Bio il 10 concentrations
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Il 10 Concentrations, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+Cytokines/pm41615539-77-2-12?v=Boster+Bio
Average 94 stars, based on 1 article reviews
il 10 concentrations - by Bioz Stars, 2026-08
94/100 stars
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monocyte chemoattractant protein 1 mcp 1  (Boster Bio)
93
Boster Bio monocyte chemoattractant protein 1 mcp 1
FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ek0902  (Boster Bio)
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FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Ek0902, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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interleukin 10 il 10 concentrations  (Shanghai Korain Biotech Co Ltd)
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FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels <t>of</t> <t>VEGF</t> were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific <t>proteome</t> profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.
Interleukin 10 Il 10 Concentrations, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels of VEGF were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific proteome profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.

Journal: Journal of Biological Chemistry

Article Title: Xanthine Oxidoreductase Promotes the Inflammatory State of Mononuclear Phagocytes through Effects on Chemokine Expression, Peroxisome Proliferator-activated Receptor-γ Sumoylation, and HIF-1α

doi: 10.1074/jbc.m110.150847

Figure Lengend Snippet: FIGURE 9. Inhibition of XOR activity increases levels of HIF-1 protein in rat inflammatory MNP and in PMA-differentiated U937 cells. A, I-MNP were purified from rat lungs 24 h following insufflation of Th-1 cytokines and were plated in 12-well plates at 1.0 106 cells/well and grown under normoxic conditions. Nonadherent cells were removed by washing after 1 h, and cells were treated with the indicated doses of MIG132. Cells were harvested after 6 h; whole cell lysates were prepared and Western immunoblots run with antibody to HIF-1. In addition, washed, adherent cells were exposed to MIG132 (50 M) for the indicated times, and Western immunoblots were run on whole cell lysates and probed with antibody to HIF-1. Blots were subsequently stripped and re-probed with antibody to GAPDH to control for protein loading. B, I-MNP were purified and plated as in A. Plates were placed in either nor- moxic (21% O2, 5% CO2) or hypoxic culture (1% O2, 5% CO2, 94% N). After 1 h, cells were treated with the XOR inhibitors allopurinol (Allo, 150 M), oxypuri- nol (Oxy, 150 M), or Y-700 (50 nM). One h later cells were treated with MIG132 (50 M) and grown for 6 h. Whole cell lysates were then prepared and West- ern immunoblots run with independent duplicate samples as indicated. Blots were first probed with antibody to HIF-1 and subsequently with antibody to GAPDH. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of duplicate samples. C, I-MNP were purified, plated, and grown in normoxia in the presence of MIG132 as in A. Cells were exposed to the XOR inhibitor Y-700 (50 nM) for 1 h and subsequently treated with IL-1 (10 ng/ml), IFN- (20 ng/ml), or LPS (1.0 g/ml). Whole cell lysates were pre- pared and Western immunoblots run after 24 h of exposure to cytokines. Blots were run on triplicate samples, and representative blots are shown. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. D, U937 cells were plated in 12-well plates at 1 106 cells/well and treated with PMA (30 nM) for 48 h. Cells were then washed; the medium was replaced, and cells were grown under normoxic or hypoxic conditions for 1 h in the presence of 50 M MIG132. Subsequently, cells were treated with Y-700 (50 nM) for 1 h and cytokines added as above. Whole cell lysates were prepared after 24 h of exposure to cytokine/MIG132, and Western immunoblots were run sequentially with antibody to HIF-1 and GAPDH. Representative blots are shown of three independent blots for each experiment. Bands from Western immunoblots were quantitated by scanning dosimetry and normalized to the signal obtained from the GAPDH blots. Data show the mean S.D. of triplicate blots. E, I-MNP were purified and plated in normoxia as in B. Levels of VEGF were quantitated in the cell-free supernatant over a period of 48 h in culture. In addition, cells were treated independently with MIG132 in the absence or presence of allopurinol, oxypurinol, or Y-700 as in B, and levels of VEGF were measured in the cell-free supernatant 6 or 24 h after treatment. Data show the mean S.D. of three independent experiments. F, I-MNP were plated as above in the presence or absence of Y-700 (1.0 mM) and in the absence of MIG132. After 24 h, cell-free supernatants were collected and analyzed with the rat-specific proteome profiler. Experiments were performed in quadruplicate, and each cytokine was analyzed from two spots on each filter (boxed for VEGF), and thus all data reflect eight independent determinations for VEGF alone. Spots were quantitated as described by the supplier using an R&D transmission mode scanner and image analysis software from R&D. Data show the mean S.D. of light transmission signals (arbitrary units) from eight spots for VEGF only and both control and Y-700 groups and were normalized first to the mean positive control spots (A1, A2, A19, A20, D1, and D2) and subsequently to the signal obtained from the control samples, which was thereby set at 1.00. ***, p 0.02.

Article Snippet: VEGF was also measured using the rat specific proteome profiler array (catalog no. ARY008, R&D Systems, Inc.).

Techniques: Inhibition, Activity Assay, Purification, Western Blot, Control, Transmission Assay, Software, Positive Control

Journal: Cell Reports Medicine

Article Title: A functional cardiac patch promotes cardiac repair by modulating the CCR2 − cardiac-resident macrophage niche and their cell crosstalk

doi: 10.1016/j.xcrm.2025.101932

Figure Lengend Snippet:

Article Snippet: Rat CXCL2/MIP-2 ELISA Kit , Boster Bio. , EK0725.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Staining, Labeling, H&E Stain, Lysis, Software