Rat Cytokines Search Results


92
Quansys Biosciences rat cytokine multiplex array
Serum <t>cytokine</t> levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats <t>fed</t> <t>GEN</t> before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.
Rat Cytokine Multiplex Array, supplied by Quansys Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems proteome profiler rat xl cytokine array kit
Serum <t>cytokine</t> levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats <t>fed</t> <t>GEN</t> before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.
Proteome Profiler Rat Xl Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems ary008
Serum <t>cytokine</t> levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats <t>fed</t> <t>GEN</t> before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.
Ary008, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems panel a
Serum <t>cytokine</t> levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats <t>fed</t> <t>GEN</t> before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.
Panel A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems rat xl cytokine array
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Rat Xl Cytokine Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio il 10 concentrations
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
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Boster Bio elisa kits
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Boster Bio il 10
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Il 10, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio monocyte chemoattractant protein 1 mcp 1
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Monocyte Chemoattractant Protein 1 Mcp 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio cxcl1
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Cxcl1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio ek0902
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Ek0902, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quansys Biosciences human cytokine strip wells 16 plex assay
Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory <t>cytokine</t> gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.
Human Cytokine Strip Wells 16 Plex Assay, supplied by Quansys Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Serum cytokine levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats fed GEN before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Lifetime genistein intake increases the response of mammary tumors to tamoxifen in rats

doi: 10.1158/1078-0432.CCR-16-1735

Figure Lengend Snippet: Serum cytokine levels of (A) IL-1α, (B) IL-6 and (C) IL-12 and tumor mRNA levels of (D) Treg cell marker Foxp3 and (E) its down-stream target TGFβ1, and (F) cytotoxic T cell marker CD8a in rats fed GEN before puberty, adulthood or both and not yet treated with tamoxifen (TAM) (n=9 adenocarcinomas per group). GEN intake, either past or current, reduced Foxp3 levels and increased CD8a levels. Same end-points were measured in tumors during TAM treatment (G, H and I) and this assessment included tumors from rats that started GEN intake for the first time during TAM treatment (n=4–6 partially responding or de novo resistant adenocarcinomas per group). Lifelong GEN intake reduced Foxp3 and increased CD8a levels in TAM treated rats. Data are presented as means ± SEM; bars with different letters are significantly different from each other, p<0.05. *significantly different from no GEN control, #statistically different from post-diagnosis GEN exposed rats, p<0.05.

Article Snippet: To investigate possible changes in serum cytokine levels in rats fed GEN at different times of their life, a rat cytokine multiplex array (#110449RT) was carried out by Quansys Biosciences (Logan, UT).

Techniques: Marker, Control, Biomarker Discovery

Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory cytokine gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.

Journal: Bioactive Materials

Article Title: Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement

doi: 10.1016/j.bioactmat.2025.05.005

Figure Lengend Snippet: Evaluation of gene and protein expression related to extracellular matrix (ECM), cell-cell, cell-ECM connection, various secretion factors, and angiogenesis. (A) Fluorescence images of Islet and Hislet stained with collagen 1 (Col 1), laminin, connexin 43 (Con43), and E-cadherin (E-cad) (green). DAPI (blue) was stained for nucleus in entire cells and DiI (red) used for labeling the ADSC (scale bar = 100 μm). (B) Comparison of protein expression in each group as evaluated by Western blot analysis (n = 3, ∗P < 0.05 compared to Islet). Relative (C) angiogenesis and (D) anti-inflammatory cytokine gene expression result compared to Islet group evaluated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR, n = 3, ∗P < 0.05 compared to Islet). (E) Images of cytokine array result of collected conditioned media (CM) collected from each group and (F) quantification of the cytokine array. (G) Image of tube formation assay after treating CM from each group to human umbilical vein endothelial cells (HUVEC, 6 h treatment, n = 4 scale bar = 400 μm). (H) Quantification of tube, node, mesh after HUVEC tube formation in each group (n = 4). (I) Quantification of closed area of HUVEC after migration (n = 4). Data are presented as the mean ± SD.

Article Snippet: After retrieving the conditioned media (CM) from each group, RAT XL cytokine array (ARY030, R&D Systems) was conducted following the manufacturer's protocols.

Techniques: Expressing, Fluorescence, Staining, Labeling, Comparison, Western Blot, Gene Expression, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Tube Formation Assay, Migration